Dr. Deisseroth focused on microbial light-activated proteins such as channelrhodoposin of green algae and spearheaded “optogenetics”—a new methodological discipline in which neurons can be activated or inhibited on the millisecond scale using light. This achievement has revolutionized the field of systems neuroscience, enabling causal study of neuronal assembly activity and resulting function, beyond correlational studies.
Dr. Karl Deisseroth has spearheaded “optogenetics,” a new methodological discipline in which cellular activity is controlled by light, and has revolutionized systems neuroscience research. As a result, he has successfully developed a novel and universal technique to manipulate brain activity at the circuit level and causally manipulate dynamic cellular ensemble activities to demonstrate their necessity and sufficiency in brain functions.
Before optogenetics, systems neuroscience research was primarily based on correlation between neuronal activities and animal behaviors. To address their causality, a variety of techniques were tried also in conventional methodologies, however, the optogenetic method has been transformative in that it can activate and inhibit, with a time resolution of milliseconds, activities of specific neurons identified by molecular markers. He has, to date, made three essential contributions to the field of optogenetics.
Firstly, he demonstrated that channelrhodopsin-2, a light-activated ion channel of green algae, is a molecular device that can control action potential firing in the mammalian brain on the millisecond scale (1). This proved to be a groundbreaking discovery. Successively, he and his colleagues improved in optogenetically evoking and suppressing neural firing by modifying the molecule, or through developing new actuator molecules (2–5, see also 6, 7). Furthermore, by making full use of genetic engineering , he made it possible to study neuronal circuit dynamics in brain regions of interest in vivo (8, 9). More than a thousand laboratories around the world thus begun to perform manipulative interrogation of neuronal circuits. The optogenetics led to more frequent use of rodents as animal models in systems neuroscience research.
Secondly, by utilizing the capability of optogenetics to artificially trigger brain activity, he and his collaborators implemented optical intervention as a means for elucidating higher brain functions, such as learning and memory (10), fear and anxiety (11), reward and pleasure (9), as well as decision-making (12). Although there is no guarantee that all the optogenetically-inducible neuronal activities correspond to naturally-occurring brain activities, optogenetics has demonstrated that a particular activity pattern of neuronal ensembles is sufficient for the execution of a particular brain function, thus providing a novel framework for a causal systems neuroscience.
Thirdly, he obtained data which reinforce the hypothesis that the pathological basis of various neurodegenerative diseases and psychiatric disorders lies in the malfunction of neuronal circuit activity. This consequently led to recent expansion of CNS drug screen targets from “specific molecular pathways” to “pathology-associated neuronal circuit activities”, paving the way for new drug discovery (13, 14).
Dr. Deisseroth not only led innovative research during the early period of the optogenetics but also shared the resources or experimental protocols with the research community and intensively disseminated them. The results of his research have been published in numerous journals, and he has been honored with many awards. On his own, Dr. Deisseroth has also co-founded a drug discovery startup focusing on the utilization of the optogenetics. He also organizes many workshops or training courses in his laboratory, where he welcomes young researchers aspiring to study optogenetics. All these efforts have helped spread the technological advance and have tremendously contributed to pushing the frontiers of neuroscientific research.
For these reasons, the Inamori Foundation is pleased to present the 2018 Kyoto Prize in Advanced Technology to Dr. Karl Deisseroth.
(1) Boyden ES, et al. (2005) Millisecond-timescale, genetically targeted optical control of neural activity. Nat Neurosci 8: 1263–1268.
(2) Zhang F, et al. (2007) Multimodal fast optical interrogation of neural circuitry. Nature 446: 633–639.
(3) Gradinaru V, et al. (2007) Targeting and readout strategies for fast optical neural control in vitro and in vivo. J Neurosci 27: 14231–14238.
(4) Gradinaru V, et al. (2008) eNpHR: A Natronomonas halorhodopsin enhanced for optogenetic applications. Brain Cell Biol 36: 129–139.
(5) Gunaydin LA, et al. (2010) Ultrafast optogenetic control. Nat Neurosci 13: 387–392.
(6) Han X & Boyden ES (2007) Multiple-color optical activation, silencing, and desynchronization of neural activity, with single-spike temporal resolution. PLoS One 2: e299.
(7) Chow BY, et al. (2010) High-performance genetically targetable optical neural silencing by light-driven proton pumps. Nature 463: 98–102.
(8) Sohal VS, et al. (2009) Parvalbumin neurons and gamma rhythms enhance cortical circuit performance. Nature 459: 698–702.
(9) Tsai HC, et al. (2009) Phasic firing in dopaminergic neurons is sufficient for behavioral conditioning. Science 324: 1080–1084.
(10) Liu X, et al. (2012) Optogenetic stimulation of a hippocampal engram activates fear memory recall. Nature 484: 381–385.
(11) Tye KM, et al. (2011) Amygdala circuitry mediating reversible and bidirectional control of anxiety. Nature 471: 358-362.
(12) Zalocusky, KA et al. (2016) Nucleus accumbens D2R cells signal prior outcomes and control risky decision-making. Nature 531: 642–646.
(13) Kravitz AV, et al. (2010) Regulation of parkinsonian motor behaviours by optogenetic control of basal ganglia circuitry. Nature 466: 622–626.
(14) Yizhar O, et al. (2011) Neocortical excitation/inhibition balance in information processing and social dysfunction. Nature 477: 171–178.
My path to Kyoto began at Harvard, in the 1980s, where I first planned to focus on writing, as my calling and career. I found myself derailed by science however, becoming captivated by new principles emerging from biology and engineering. After completion of my M.D., Ph.D. and psychiatry training at Stanford (where I continue to treat patients), I launched a laboratory effort in Stanford’s Bioengineering Department, and began to build new classes of tools for studying the brain, seeking to find new answers—or at the very least, to find ways to ask new questions. New ways to ask questions are needed; our brains are biological objects, organs built from cells and blood; but in psychiatric illness, the organ itself is not damaged in a way we can see or understand now, in the way we can for a broken leg or a weakened heart.
One of the first technologies I developed, optogenetics, involves putting genes from ancient forms of bacteria and from green algae, into specific brain cells of mammals. A strange thing to do—but with a certain logic, for the microbial genes we borrowed in this way directed the production of proteins that turn light into electricity—and electricity is the fundamental language of the brain. These channelrhodopsin proteins are light-gated ion channels that enable algae to seek light conditions suitable for photosynthesis; we have been able to discover principles of function by solving the protein structures and by redesigning for altered ion selectivity, kinetics, and spectral properties. These discoveries not only revealed basic principles governing operation of light-gated ion channels for algal behavior, but also enabled the creation of new proteins for illuminating animal behavior. As a result, we can now flash laser light (delivered through thin fiberoptics deep into the brain) to turn neurons on or off, and observe the effects on behavior.
As a result of this work, we know now which cells and connections across the brain actually control key behaviors of pleasure, reward, social interaction, and motivation to meet challenges, as well as (on the negative side) symptoms of anxiety, depression and fear. This optogenetic technology has taken hold throughout neuroscience and now enables us to test precise cellular basis for behavior by controlling the brain’s circuitry.