1996 Kyoto Prize Laureates

Basic Sciences

Life Sciences(Molecular Biology, Cell Biology, Neurobiology)

Mario Renato Capecchi

/  Molecular Geneticist

1937 -

Professor, The University of Utah

Commemorative Lectures

The Making of a Scientist

1996

11 /11 Mon

Place:Kyoto International Conference Center

Workshop

From Genes to Cellular Functions and Body Plan

1996

11 /12 Tue

13:10 - 17:20

Place:Kyoto International Conference Center

Achievement Digest

Production of Knockout Mice through the Development of Gene Targeting Technique and Outstanding Contribution to Elucidation of Gene Function

A molecular geneticist who developed the “gene targeting” technique, which is widely used around the world today. With his success in breeding mice in which targeted genes were inactivated, referred to as “knock-out” mice, Dr. Capecchi established a new avenue for researching how genes work. His method of research has benefited immensely such branches of life sciences as biology, medicine and agriculture.

Citation

Dr. Mario Renato Capecchi established a new avenue for researching how a gene functions by successfully breeding “knocked-out” mice in which the targeted gene was inactivated. His method of research has since been used around the world, and has immensely benefited the fields of biology, medicine and other branches of the life sciences.

In studying the function of a gene in medicine or biology, it is most useful to prepare a mutant of the gene and study that mutant’s behavior and function. Dr. Capecchi discovered that when a gene with partly mutated DNA molecules is inserted into an animal cell, the gene is farely integrated at the matching location of a chromosome in a process known as homologous recombination. He thus proceeded to devise a method for increasing those cells in which homologous recombination had occurred, while eliminating those cells which exhibited non-homologous recombination. Referred to as “gene targeting,” this technique enables researchers to replace any gene in the cells of a vertebrate with a gene containing desired mutations, thereby enabling the function of that gene to be determined. This revolutionary technique represents a dream come true for countless researchers and a major breakthrough in medicine, biology and the life sciences.

With this gene-targeting technique, Dr. Capecchi went on to establish an efficient method of producing “knocked-out” mice, in which targeted genes are inactivated. The practical applications of “knocked-out” mice have opened broad new areas of research in embryology, helping to elucidate the functions of genes which encode receptors and other cellular components involved in functions such as signal transduction in vertebrates. Currently, this technique is actively being used to produce diseasemodel animals in order to develop therapies for hereditary, neoplastic, and other diseases. The technique also has boundless potential in plant and animal breeding and other applications. Accordingly, Dr. Capecchi’s work may be said to have enormous impact on our society as well as the research fields of academia.

Through these achievements, Dr. Mario Renato Capecchi revolutionized the study of biological phenomena, contributing greatly to the dramatic development of the life sciences.

Therefore, the Inamori Foundation is pleased to bestow upon Dr. Capecchi the 1996 Kyoto Prize in Basic Sciences.

Profile

Biography
1937
Born in Verona, Italy
1961
B.S. Antioch College, U.S.A.
1967
Ph.D. Harvard University
1968
Assistant Professor, Harvard Medical School
1973
Professor of Biology, University of Utah
1988
Investigator of the Howard Hughes Medical Institute
1989
Professor of Human Genetics, School of Medicine, University of Utah
Selected Awards and Honors
1969
American Chemical Society Awards in Biochemistry
1974
American Cancer Society Faculty Research Award
1986
Chairman of the Gordon Conference on Molecular Genetics
1991
Member of the National Academy of Sciences, U.S.A.
1993
Gairdner Foundation International Award for Achievements in Medical Sciences, Canada
1994
Sloan Prize, General Motors Cancer Research Foundation, U.S.A.
Major Works
1980
High efficiency transformation by direct microinjection of DNA into cultured mammalian cells. Cell, 22, 1980.
1987
Site-directed mutagenesis by gene targeting in mouse embryo-derived stem cells. (with K.R. Thomas) Cell, 51, 1987.
1988
Disruption of the proto-oncogene int-2in mouse embryo-derived stem cells: a general strategy for targeting mutations to non-selectable genes. (with S.L.Mansour and K.R. Thomas) Nature, 336, 1988.
1991
Regionally restricted developmental defects resulting from targeted disruption of the mouse homeobox gene hox-1.5 (with O. Chisaka) Nature, 350, 1991.
1995
Absence of radius and ulna mice lacking hoxa-11 and hoxd-11. (with A.P. Davis, others) Nature, 375, 1995.

Profile is at the time of the award.